Administration of Coumestrol and/or Dimethyl Sulfoxide Affects Vaginal Epithelium and Sex Hormones in Female Dog

Background: The overpopulation of free dogs represents a risk to public health in some countries. However, current strategies for its control are insufficient; it is convenient devise new alternatives. Methods: To determine the effects single oral administration coumestrol (COU) on vaginal epithelial cells, serum progesterone and estradiol levels clinical parameters: periovulatory bitches received biscuit that contained 600 µg COU/kg diluted DMSO or with alone without additives. Each group was subdivided into animals had ovulated day 0 those not. Result: In ovulated, COU did not affect hormonal profiles. contrast, ovulate showed lower circulating P4 concentrations days 21 28 after treatment than group. Furthermore, reduced parabasal cells increased superficial anucleate cells. estrogenic their actions depend ovulatory status animals.